Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies
Fig 1
Structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to the ACE2 ectodomain.
(A) Density map for the overall complex at the end of global structure refinement. The 3 spike protein protomers are colored in cyan, purple, and yellow, with the density for the strongly and weakly bound ACE2 proteins in pale red and green, respectively. (B) Improved density map at the contact zone between the receptor binding domain (RBD) and the strongly bound ACE2 protein ectodomain. (C) Visualization of density at the contact zone for Y501 in the RBD and residues Y41 and K353 in ACE2. (D). Ribbon diagram with superposition of the unmutated and N501Y RBD–ACE2 complex (PDB ID 7KMB). (E) Zoomed-in view of the interface, showing a superposition of the structures of unmutated and N501Y mutant spike proteins in complex with ACE2. The carbon atoms of residues in the N501Y mutant and ACE2 in our structure are colored in cyan and pale red, respectively, while those in the structure of the complex between unmutated spike protein and ACE2 are in light gray.