Strigolactone signaling regulates specialized metabolism in tobacco stems and interactions with stem-feeding herbivores
Fig 5
Increased phenolamide accumulations in SL-RNAi RSJ are positively related to JA signaling via MYB8.
(A) Relative contents of phenolamides such as CP and DCS in the RSJs of indicated plants (±SE, n = 6). (B) Relative contents of CP and DCS in RSJs of EV and myb8 plants after attack. (C) Biomass of T. m. larvae after attacking EV and myb8 plants for 2 weeks (n = 15). (D) Relative transcript abundance of MYB8 and DH29 in RSJ of EV and aoc plants (±SE, n = 4). (E) Relative contents of CP and DCS in the pith of EV and aoc plants without (Control) or with T. m. attack for 2 weeks. (F) Relative transcript abundance of MYB8 and DH29 in the RSJs of indicated plants (±SE, n = 4). (G) Relative content of CP and DCS in the pith of EV and max2#2 plants without (Control) or with T. m. attack for 2 weeks (±SE, n = 6–10) (*P < 0.05; **P < 0.01; ***P < 0.001; two-tailed Student t test). Values for graphs (A–G) are listed in S1 Data. aoc, allene oxide cyclase; ccd, carotenoid cleavage dioxygenase; CP, N′-caffeoylputrescine; d14, dwarf14; DCS, N′, N″-decaffeospermidine; DH29, ACYLTRANSFERASE DH29; EV, empty vector; JA, jasmonate; kai2, karrikin insensitive 2; max2, more axillary growth 2; n.s., not significant; RNAi, RNA interference; RSJ, root–shoot junction; SL, strigolactone; T. m., T. mucorea.