The lipidome of primary murine white, brite, and brown adipocytes—Impact of beta-adrenergic stimulation
Fig 5
Effects of β-adrenergic stimulation in brite adipocytes.
(A) LPC, (B) LPC/PC, (C) LPC composition, (D) LPC distribution, (E) LPC 16:0, (F) LPC 18:0, (G) LPC 16:1, (H) LPC 18:1; primary cells were treated with 0.5 μM ISO for 1 hour, 2 hours, or 4 hours. (I) U-13C-palmitate to–palmitoleate desaturation, (J) U-13C-palmitate to–stearate elongation; primary cells were simultaneously treated with 0.5 μM ISO and 100 μM U-13C-pamitate for 1 hour. (K) LPC, (L) LPC/PC; primary cells originating from UCP1 KO mice and WT littermates stimulated for 1 hour with 0.5 μM ISO. (M) D9-PC (N) D9-PC/total PC, (O) D9-PC composition, (P) D9-PC distribution, (Q) D9-PC 32:0, (R) D9-PC O-32:0; cells were simultaneously incubated with D9-choline and 0.5 μM ISO for 1 hour. Shown are means ± SD of 3 mice, the p-value indicates a significant difference between the treatment groups “Co” and “ISO”; annotation of “a, b” indicates that group "a" is statistically different from "b," which was determined for panels (A–B) and (E–H) using a two-way ANOVA (Post Hoc: Tukey Test) and was determined for (C–D) and (I–R) using a Student t test. The underlying data of (A–R) can be found in S1 Data. Co, control; ISO, isoproterenol; KO, knock out; LPC, lyso-PC; MUFA, monosaturated fatty acid; PC, phosphatidylcholine; PUFA, polyunsaturated fatty acid; SAFA, saturated fatty acid; UCP, uncoupling protein; WT, wild type.