Spatiotemporal dynamics of odor responses in the lateral and dorsal olfactory bulb
Fig 5
The lOB displays mechanosensitive activation beyond breathing.
(A) Raw traces (% ΔF/F) of two glomerular breathing responses during presentation of clean air flow rates (0.5, 0.25, 0.1, 0.05, and 0.005 L/min) and vacuum rates (2.5, 1.25, and 0 L/min) and room air with no external flows (1 animal, 2 glomeruli). These are presented as examples of the trial stimulus; green represents a glomerulus in the dorsal region of the lOB, blue in the ventral (maps shown in panel B). Red bar illustrates the period in which only the air is presented (“Vacuum off”), and grey is when the vacuum is also turned on (“Vacuum on”); height indicates flow rates relative to other trials. Vertical blue bars indicate response and reference frames to compute response maps. (B1, vacuum off response; B2, vacuum on response; maps shown in panel B). (B) Activation map in response to inspiration during only clean air flow rate of 0.5 L/min (Left top) and additionally the vacuum 2.5 L/min (Left bottom). Arrows indicate the ROI displayed in panel A. B1 and B2 indicate the respective time points in panel A (0.5 L/min airflow: vacuum 2.5 L/min). Right: activation map of all glomeruli chosen to clean air flow rate of 0.5 L/min (Top) and vacuum 2.5 L/min (Bottom). (C) The z-scores of the lOB glomerular responses during only clean air flow (red) and also vacuum (grey), organized from dorsal to ventral, for different clean air flow rates (0.5, 0.25, 0.1, 0.05, and 0.005 L/min) and vacuum rates (2.5, 1.25, and 0 L/min) and room air (3 animals, 47 glomeruli). Linear correlation fits are indicated. (D) Top left: histogram of the ratio of vacuum off responses relative to room air breathing responses in the lateral bulb. Top right: histogram of the ratio of vacuum on breathing responses relative to room air breathing responses in the lateral bulb (3 animals, 47 glomeruli, total 329 glomerular responses across all flow rates). Number of responses (n) was divided into number of glomerular responses <1.6 and >1.6 times above the room air breathing response. Bottom: same as top but in the dorsal bulb. Underlying data for this figure can be found in S1 Data. lOB, lateral olfactory bulb; OB, olfactory bulb; ROI, region of interest.