Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli
Fig 7
Introducing chemical diversity through supplementation and substitution of secondary tailoring enzymes.
A schematic representation of an AntA-I plug-and-play biosynthetic scaffold extended with enzymes sourced from a range of organisms. Metabolites produced through addition of O-methyltransferase (from M. truncatula) and RadH (from Chaetomium chiversii) are shown in red and blue boxes correspondingly. Enzymes within AntA-I plug-and-play core biosynthetic pathway are represented in light grey boxes. Additional non-cognate secondary tailoring enzymes are shown in darker grey boxes. Dotted lines represent deviation away from the natural AQ256 biosynthetic pathway. ACP, acyl carrier protein; CLF, chain length factor; KS, ketosynthase.