CD71+VISTA+ erythroid cells promote the development and function of regulatory T cells through TGF-β
Fig 7
The cross-talk between Tregs and CD71+ erythroid cells.
(A) Representative flow cytometry dot plot of Tregs in neonatal FOXP3-DTR mice either control or treated with DT. (B) Representative dot plots showing percent CD71+ erythroid cells in control versus DT-treated FOXP3-DTR mouse. (C) Percentages of CD71+ erythroid cells in control versus DT-treated mice. (D) Representative dot plots showing percent CD71+ erythroid cells in control versus DT-treated WT mice. (E) Percentages of CD71+ erythroid cells in control versus DT-treated WT mice. (F) Representative histogram plots showing expression of VISTA on CD71+ erythroid cells in control versus DT-treated mice. (G) Cumulative data showing MFI of VISTA on CD71+ erythroid cells in control versus DT-treated mice. (H) Representative histogram plots showing expression of CD71 on CD71+ erythroid cells in the presence and absence of Tregs. (I) Cumulative data showing MFI of CD71 on CD71+ erythroid cells alone or when cocultured with Tregs in vitro. (J) Representative histogram plots showing Ki67 expression on CD71+ erythroid cells in the presence and absence of Tregs. (K) Cumulative data showing MFI of Ki67 on CD71+ erythroid cells alone or upon coculture with Tregs in vitro. (L) Representative dot plots showing expression of VISTA on human cord blood and placenta CD71+ erythroid cells. (M) Cumulative data showing percentages of VISTA+ cells among human cord blood and placenta CD71+ erythroid cells. (N) Data showing PD-1H gene expression in enriched cord blood and placenta CD71+ erythroid cells. (O) Data showing TGF-β gene expression in human cord blood and placenta CD71+ erythroid cells. The underlying data can be found in S1 Data. CD71, cell-surface transferrin receptor; DT, diphtheria toxin; DTR, DT receptor; FOXP3, forkhead box P3; FOXP3-DTR, FOXP3 mice expressing DTR in Treg cells; Ig, immunoglobulin; Iso., isotype; Ki67, antigen KI67; MFI, mean fluorescence intensity; PD-1H, Programmed Death-1 Homologue-1; TER119, glycophorin A-associated protein, an erythroid-specific antigen expressed on erythrocytes; TGF-β, transforming growth factor beta; Treg, regulatory T cell; VISTA, V-domain Ig Suppressor of T Cell Activation; w, with; WT, wild type; w/o, without.