CD71+VISTA+ erythroid cells promote the development and function of regulatory T cells through TGF-β
Fig 5
Comparison of CD71+ erythroid cells in VISTA KO to their counterparts in WT mice.
(A). Representative plots showing frequency of CD71+ erythroid cells in a WT versus VISTA KO newborn mouse (day 9). (B) Cumulative data comparing the percentages of CD71+ erythroid cells in days 6 and 9 of WT versus KO mice. (C) Representative flow plots showing TGF-β production by a WT versus KO newborn mouse. (D) Cumulative data showing percentages of TGF-β–producing cells among CD71+ erythroid cells in WT and VISTA KO mice. (E) Representative flow cytometry dot plots showing percentages of iTregs in the presence of naïve T cells; CD71+VISTA KO, CD71+VISTA−, or CD71+VISTA+ erythroid cells; and re. TGF-β. (F) Cumulative data showing percentages of change in CD4+FOXP+ Tregs in the presence of either CD71+VISTA KO, CD71+VISTA+, or CD71+VISTA− erythroid cells and re. TGF-B. (G) Representative flow plots showing percentages of Tregs in WT and VISTA KO newborn mice (day 9). (H) Cumulative data showing percentages of Tregs in WT versus VISTA KO day 9 mice. (I) Representative plots showing expression of GARP on CD71+ erythroid cells in WT and VISTA KO day 9 mice. (J) Cumulative data showing percentages of CD71+GARP+ cells in WT versus VISTA KO day 9 mice. (K) Representative flow plots showing CD71+TER119− cells in WT versus VISTA KO newborns, and representative plot showing percent CD3+ T cells among CD71+TER119− cells in VISTA KO mouse. (M) Cumulative data showing percentages of CD71+TER119− cells in WT and VISTA KO mice (6 and 9 days old). Each point represents data from an individual mouse, representative of at least two independent experiments. Bar, mean ± one standard error. The underlying data can be found in S1 Data. CD71, cell-surface transferrin receptor; GARP, glycoprotein A repetitions dominant; Ig, immunoglobulin; iTreg, induced Treg; KO, knock-out; re. TGF-β, recombinant TGF-β; TER119, glycophorin A-associated protein, an erythroid-specific antigen expressed on erythrocytes; TGF-β, transforming growth factor beta; Treg, regulatory T cell; VISTA, V-domain Ig Suppressor of T Cell Activation; WT, wild type.