CD71+VISTA+ erythroid cells promote the development and function of regulatory T cells through TGF-β
Fig 2
CD71+ erythroid cells express VISTA.
(A) Representative dot plots showing VISTA expression by CD71+ erythroid cells from a day 3 newborn mouse. (B) Percentages of CD71+VISTA+ erythroid cells among splenocytes of BALB/c, (C) C57BL/6, and (D) F1 mice are shown at different ages, respectively. (E) Representative histogram showing VISTA expression (red line) or isotype control (black line) and (F) the MFI of VISTA among CD71+ erythroid cells in BALB/c mice. (G) Representative histogram showing VISTA expression (red line) or isotype control (black line) and (H) the MFI of VISTA among CD71+ erythroid cells in B57BL/6 mice. (I) Representative histogram showing VISTA expression (red line) or isotype control (black line) and (J) the MFI of VISTA among CD71+ erythroid cells in F1 mice. (K) RT-PCR of VISTA gene expression among neonatal CD71+ erythroid cells in BALB/c mice at different ages. (L) Representative histogram of VISTA expression among neonatal CD71+ erythroid cells originated from either spleen or BM. (M) The MFI for VISTA among neonatal CD71+ erythroid cells from spleen versus BM of neonatal mice. Each point represents data from an individual mouse, representative of at least 2–3 independent experiments. Bar, mean ± one standard error. The underlying data can be found in S1 Data. BM, bone marrow; CD71, cell-surface transferrin receptor; F1, filial 1 hybrid mice; Ig, immunoglobulin; Iso., isotype; MFI, mean fluorescence intensity; RT-PCR, reverse transcription PCR; TER119, glycophorin-A–associated protein; VISTA, V-domain Ig Suppressor of T Cell Activation.