Classes and continua of hippocampal CA1 inhibitory neurons revealed by single-cell transcriptomics
Fig 9
Analysis of Cxcl14 co-expression patterns confirms predicted properties Cck.Cxcl14 cells.
(A) Cxcl14-expressing cells are CGE derived: in situ hybridization for Cxcl14 combined with immunohistochemistry for YFP in the Lhx6-Cre/R26R-YFP mouse yields no double labeling. (B) Double in situ hybridization for Cxcl14 and Reln marks a population of neurons located primarily at the sr/slm border. Note Reln expression without Cxcl14 in so and slm, likely reflecting O-LM and neurogliaform cells. (C–E) Subsets of the Cxcl14-positive neurons are positive for pro-CCK or CALB1 (in situ hybridization plus immunohistochemistry), or Npy (double in situ hybridization). (F, G) No overlap was seen of Cxcl14 with Nos1 or Kit. In all panels, arrowheads indicate double-expressing neurons. Scale bars: 200 μm (A), 100 μm (B–G). b, sr/slm border region; O-LM, oriens/lacunosum-moleculare; slm, stratum lacunosum-moleculare; sm, stratum moleculare of the dentate gyrus; so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum; sr/slm, stratum radiatum and stratum lacunosum-moleculare; YFP, yellow fluorescent protein.