Classes and continua of hippocampal CA1 inhibitory neurons revealed by single-cell transcriptomics
Fig 5
Analysis of discrete versus continuous variation by negative binomial discriminant analysis.
(A) Histogram of log-likelihood ratios for three example cluster pairs, measuring how much better each cell’s whole-genome expression pattern is explained by one or the other cluster. The top histogram (basket versus axo-axonic cells) is clearly bimodal, indicating discrete separation. The bottom two histograms (ivy versus MGE-neurogliaform cells; two subclasses of Cck/Cxcl14 cells) show substantial overlap, indicating continuous variation between clusters. The degree of bimodality is captured by the d’ statistic above each plot. (B) Pseudocolor matrix showing continuity of each pair of clusters, as assessed by d’ statistic. White means strongly bimodal; darker colors indicate continuity.