Low-intensity electromagnetic fields induce human cryptochrome to modulate intracellular reactive oxygen species
Fig 2
PEMF response of HEK293 cell cultures.
(A) Cells of WT or HSCRY1 and HSCRY2 double KD (HsCry) were seeded in 24-well cell culture dishes and grown for 48 hours in the presence (PEMF, black bars) or absence (Sham, white bars) of applied PEMF as described (Materials and methods). The concentration of H202 in the culture media was determined using the Amplex Red fluorescent detection system (Materials and methods) and normalized to the number of cells. The graph presents the relative concentration of H202 from PEMF-treated cells (PEMF, black bars) compared to the control (Sham, white bars) untreated sample. Error bar represents SD of 12 independent measurements. (B) Relative number of cells per well in PEMF-treated cells (PEMF, black bars) compared to the control (Sham, white bars) untreated sample. n = 12 biological repeats. Error bar represents SD. ***p < 0.001 (see Materials and methods). Underlying data is included in S1 Data. HEK293, human embryonic kidney cells; HsCry, human cryptochrome; KD, knockdown; PEMF, pulsed electromagnetic field; WT, wild-type.