Insight into small molecule binding to the neonatal Fc receptor by X-ray crystallography and 100 kHz magic-angle-spinning NMR
Fig 4
Triple-resonance MAS NMR spectra enable assignments of chemical-shifts.
Sequential resonance assignments using the experiments (H)CANH (blue), (H)CA(CO)NH (red), and (H)CBCANH (green) recorded on fully protonated [13C,15N]-labeled FcRnECD at 100 kHz MAS. As an example, the sequential connections from K41β2m to R45β2m in β2m are indicated by dashed lines. All assigned chemical-shifts can be found in S1 Table, S2 Data, and in the BMRB (accession number 27437). β2m, β2-microglobulin; BMRB, Biological Magnetic Resonance Data Bank; FcRnECD, extracellular domain of the neonatal Fc receptor; MAS, magic-angle-spinning.