Insight into small molecule binding to the neonatal Fc receptor by X-ray crystallography and 100 kHz magic-angle-spinning NMR
Fig 3
Proton-detected MAS NMR on fully protonated FcRnECD.
(A) The soluble FcRnECD (42 kDa) was sedimented by ultracentrifugation at 100,000 x g directly into a 0.7 mm MAS NMR rotor using a home-made filling tool. (B) 2D 15N-1H correlation spectrum recorded at 100 kHz MAS of fully protonated [13C,15N]-labeled FcRnECD. (C) Typical linewidths of 1H (1) and 15N (2) at full-width-half-maximum (FWHM) of a selected cross peak from the 15N-1H spectrum. β2m, β2-microglobulin; FcRn, neonatal Fc receptor; FcRnECD, extracellular domain of the neonatal Fc receptor.