Morphology of mitochondria in spatially restricted axons revealed by cryo-electron tomography
Fig 4
Structural features of a mitochondrion captured spanning 2 varicosities (“Var”).
(A) A 10-nm slice through a 3D tomographic reconstruction showing a mitochondrion spanning 2 closely spaced varicosities connected by a short (approximately 100 nm) axon segment. An expanded region of the red box shown in (A) reveals the tubulated nature of the portion of the mitochondrion within the axon segment. Microtubules can be seen running parallel to the tubulated portion of the mitochondrion. (B) shows a surface rendered version highlighting the plasma membrane (purple), microtubules (blue), small segment of ER (yellow), and mitochondria (green). (C) is the same mitochondrion as in (B), displaying distinct segmentation of the outer membrane (green), inner membrane (orange), and cristae (pink).