Morphology of mitochondria in spatially restricted axons revealed by cryo-electron tomography
Fig 2
Tomographic reconstruction of a typical presynaptic varicosity and adjoining axon segment.
(A) A 2D slice from the tomographic reconstruction showing the distribution of organelles in the varicosity (Mito = mitochondrion). Scale bar = 200 nm. (B) Segmented representation of the entire 3D tomogram volume shown in (A) revealing the relative size and spatial distribution of the organelle environment in the varicosity and axon segment. PM (dark blue), MT (light blue), mitochondrial outer membrane (“Mito,” dark green), ER (yellow), Ves (dark purple), MVB (light purple), unidentified membrane-bound compartment (pink). Scale bar = 200 nm. ER, endoplasmic reticulum; MVB, multivesicular body; MT, microtubules; PM, plasma membrane; Ves, vesicle.