KDM5 histone demethylases repress immune response via suppression of STING
Fig 4
Inhibition of KDM5 demethylases induces STING expression.
(A, B) RT-qPCR analysis (panel A) or western blot (panel B) analysis of the indicated cells after treatment with DMSO or 1 μM KDM5-C70 for 3 days. (C) Western blot analysis of MCF7 cells transfected with the indicated expressing plasmids. (D–F) RT-qPCR (panel D and E) or western blot (panel F) analyses of MCF7 cells with knockout of indicated genes after treatment with DMSO or 1 μM KDM5-C70 for 6 days. (G) Western blot analysis of MCF7 cells 2 days after transfection with different amounts of the indicated plasmid. (H–L) Western blot (panel H) or RT-qPCR (panel I–L) analysis of MCF7, SKBR3, or BT474 cells after treatment with DMSO or 1 μM KDM5-C70 for the indicated length of time. Representative data from triplicate experiments are shown. Error bar denotes SEM. #p < 0.01 for inhibitors versus DMSO (panel A, D, E, and I–L). The numerical values used to generate graphs in panel A, D, E, and I–L are available in S1 Data. RT-qPCR, reverse transcription followed by quantitative PCR; STING, stimulator of interferon genes.