Identifying novel strategies for treating human hair loss disorders: Cyclosporine A suppresses the Wnt inhibitor, SFRP1, in the dermal papilla of human scalp hair follicles
Fig 2
SFRP1 modulates canonical β-catenin activity at the ligand level in the human HF bulb ex vivo.
(A–F) The human HF bulb expresses the core components of the canonical β-catenin pathway. Immunofluorescence of β-catenin (A), in situ hybridisation of AXIN2 (B), LEF1 (C), and WLS (D). (G–O) qRT-PCR analysis of SFRP1 and the direct β-catenin target genes AXIN2 and LEF1 in the human HF with IWP-2 (G, J, M), rhSFRP1 (H, K, N), and WAY-316606 (I, L, O) treatment (n = 4 male patient samples). (P–T) Analysis of AXIN2 mRNA by in situ hybridisation after WAY-316606 treatment (n = 15 HFs control, 14 HFs WAY-316606; from 3 male patient samples). (G–O) One-sample t test, (Q and S) two-tailed unpaired t test (R and T), and Mann-Whitney test. Data are expressed as mean ± SEM. Scale bars = 50 μm. Underlying data can be found in S1 Data. AXIN2, axis inhibition protein 2; DP, dermal papilla; HF, hair follicle; IWP-2, inhibitor of Wnt production-2; LEF1, lymphoid enhancer binding factor 1; n.s, not significant; PPIB, peptidylprolyl isomerase B; Pre-Cx, pre-cortex, qRT-PCR, quantitative real-time PCR; rhSFRP1, recombinant human SFRP1; SFRP1, secreted frizzled related protein 1; WAY, WAY-316606; WLS, Wntless.