Pharmacogenomic identification of small molecules for lineage specific manipulation of subventricular zone germinal activity
Fig 2
Connectivity map (CMAP) identification of perturbagens that promote region-specific subventricular zone (SVZ) signatures, oligodendrogenesis, and reactivation of adult neural stem cells (NSCs).
(A) Schematic representation of the experimental flow. Microarray datasets were compared for obtaining expression signatures (detailed in Materials and Methods) and uploaded onto searchable platform-independent expression database (SPIED) to interrogate the CMAP for obtaining a list of perturbagens. These were further inspected for their known protein targets, categorized and presented in the figure as pie charts (ordered clockwise following their ranking order). Note that small molecules related to distinct top-ranked categories are observed in the different analysis. Genes enriched in (B) dNSCs/dTAPs compared to other early postnatal datasets (i.e., dorsalization); (C) lNSCs/lTAPs compared to other early postnatal datasets (i.e., ventralization); (D) oligodendrocyte (OL) lineage cells compared to dNSCs and dTAPs; (E) Postnatal NSCs compared to adult NSCs. dNSCs, dorsal NSCs; dTAPs, dorsal TAPs; lNSCs, lateral NSCs; lTAPs, lateral TAPs.