Tor1 and CK2 kinases control a switch between alternative ribosome biogenesis pathways in a growth-dependent manner
Fig 5
Role of Sch9, tap42, or tip41 in the A2 to A3 switch in pre-rRNA processing.
(A) Pre-rRNA processing before and after diauxic shift in the yeast strain lacking Sch9 kinase (sch9Δ) or in the tap42-11 mutant. The OD600 values of harvested samples are shown above the lanes. Northern blot using the A2–A3 probe. (B) Pre-rRNA processing before and after diauxic shift in the strain expressing the hyperactive mutant Sch9-2D3E grown in SDC media. Samples were harvested at indicated OD600, and total RNA was analyzed by northern blotting using probe A2–A3. (C) The sch9Δ or wild-type strains were treated by rapamycin at OD600 = 2, and total RNA was isolated at indicated time points and analyzed by northern blotting (probe A2–A3). (D) The tap42-11 mutant and tip41Δ strain were treated with rapamycin as in (C).