TURAN and EVAN Mediate Pollen Tube Reception in Arabidopsis Synergids through Protein Glycosylation
Fig 6
ANX1-YFP fluorescence is not detectable in tun mutant pollen grains.
(A–D) Confocal microscope analysis of ANX1-YFP expression under a pollen-specific promoter. (A) ANX1-YFP expression in TUN/TUN;qrt/qrt;ANX1-YFP/ANX1-YFP (wild-type segregants homozygous for the reporter gene). (B) ANX1-YFP expression in TUN/TUN;qrt/qrt;ANX1-YFP/- (wild-type segregants hemizygous for the reporter gene). (C) ANX1-YFP expression in tun-2/TUN;qrt/qrt;ANX1-YFP/ANX1-YFP mutant tetrads. Arrowheads indicate missing fluorescence in tun pollen grains. (D) ANX1-YFP expression in tun-2/TUN;qrt/qrt;ANX1-YFP/ANX1-YFP mutant tetrads after Kifunensine (Kif) treatment. (E) ANX1-YFP expression in tun-2/TUN;qrt/qrt;ANX1-YFP/ANX1-YFP mutant tetrads after mock treatment for fluorescence intensity decrease comparison. (F–H) ANX1-YFP expression in tun-2/TUN;qrt/qrt;ANX1-YFP/ANX1-YFP mutant tetrads after Eeyarestatin I (EerI) treatment. (F) 10 μm EerI recovers ANX1-YFP fluorescence in tun pollen grains. (G) Higher concentrations of EerI can lead to cytosolic inclusions (asterisk) or pollen grain burst (arrow). (H) ANX1-YFP fluorescence recovery in several tun-2/TUN;qrt/qrt;ANX1-YFP/ANX1-YFP mutant tetrads after EerI treatment. Residual fluorescence signal from the pollen coat is autofluorescence. Scale bars: 20 μm.