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TURAN and EVAN Mediate Pollen Tube Reception in Arabidopsis Synergids through Protein Glycosylation

Fig 5

NTA, FER and LRE show proper localization in tun and evn mutant embryo sacs.

(A–I) Confocal microscope analysis of fluorescently labeled proteins. (A–C) Vesicle-associated NTA-GFP localization in the cytoplasm of a wild-type (A), tun-2 (B), and evn-3 FG (C). (D–F) FER-GFP at the FA and in membranes of sporophytic tissue of a wild-type (D), tun-2 (E), and evn-3 FG (F). (G–I) Extracellular localization of LRE-Citrine in a wild-type (G), tun-2 (H), and evn-3 FG (I). Scale bars: 20 μm.

Fig 5

doi: https://doi.org/10.1371/journal.pbio.1002139.g005