TURAN and EVAN Mediate Pollen Tube Reception in Arabidopsis Synergids through Protein Glycosylation
Fig 2
Distinct pollen defects in tun and evn mutants.
(A) Pollen in vitro germination assay of qrt/qrt pollen grains. (B) Pollen in vitro germination assay of tun-1/TUN;qrt/qrt pollen. Arrows indicate PT bursting. (C) Graph of PT bursting counts in qrt/qrt and tun-1/TUN;qrt/qrt pollen. (D) Pollen in vitro germination assay of evn-1/EVN;qrt/qrt mutant pollen. Arrowheads indicate degenerated pollen grains. (E) Graph of degenerating pollen grain counts at different stages of evn-1/EVN mutants after DAPI staining. Stage four refers to the bicellular and early tricellular, stage three to the tricellular, stage two to the late tricellular and early mature, and stage one to the mature pollen stage. Scale bars: 20 μm. (F–G) Gene model of TUN (F) and EVN (G) with mutant alleles. Ethane methyl sulfonate (EMS) single nucleotide polymorphisms (SNPs) are indicated by lines, T-DNA insertions by triangles.