TURAN and EVAN Mediate Pollen Tube Reception in Arabidopsis Synergids through Protein Glycosylation
Fig 1
tun and evn ovules display pollen tube overgrowth and increased callose accumulation at the filiform apparatus.
(A–C) Aniline Blue staining of callose in PT cell walls 2 d after pollination (DAP). (A) PT reception in a wild-type FG. Arrowhead indicates site of PT growth arrest. (B–C) PT overgrowth in tun-1 (B) and evn-1 mutant FGs (C). Asterisks indicate PT overgrowth. (D–F) β-glucuronidase (GUS) staining of synergid marker ET2634 2 d after emasculation (DAE) in wild-type (D), tun-1 (E), and evn-1 mutant FGs (F). Arrow indicates abnormal structure at the FA. (G–I) Chloral hydrate clearings of ovules 2 DAE in wild-type (G), tun-1 (H), and evn-1 mutants (I). Arrows indicate abnormal structure at the FA. (J–L) Aniline Blue staining of callose in 6 μm sections of wild-type (J), tun-1 (K), and evn-1 ovules 2 DAE (L). Boxes represent close-ups of indicated regions, whereby mutant close-ups in (K) and (L) were captured with reduced exposure time compared to the wild type (J). Scale bars in A–F and J–L = 20 μm; scale bars in G–I = 10 μm.