Macrophages Contribute to the Cyclic Activation of Adult Hair Follicle Stem Cells
Figure 5
Resident macrophages express HF-SC stimulatory factors before the onset of anagen.
(A) CD11b+Gr1−F4/80+ macrophages were FACS-isolated from Te and Tl backskin samples. Their mRNAs were purified and used to perform microarray analyses to evaluate changes in gene expression at Tl (P69) versus Te (P44). Histograms show a shortlist of up- and downregulated genes that have been involved in the control of HF-SC activation and apoptosis. The gating strategy is shown in Figure S3 A. (B) Relative mRNA expression of Wnt7b and Wnt10a in FACS-sorted CD11b+Gr1−F4/80+ cells at Te, Tm, Tl, and A; n = 3. The gating strategy is shown in Figure S3A. (C) Immunofluorescence staining of F4/80+ perifollicular macrophages (green). (D) Each histogram point represents the mean value of double positive F4/80+Wnt7b+ and F480+Wnt10a+ over total F4/80+ perifollicular macrophages. 10 fields/section/mouse were analyzed; n = 4. (E) Immunofluorescence of Wnt7b (green)/F4/80 (red), and Wnt10a (green)/F4/80 (red), counterstained with DAPI (blue) of skin sections at Te, Tm, Tl, and A; n = 3. Bar = 50 µm. n.s. non significant, Note: n refers to the number of mice, per point per condition. *p≤0.05. All data used to generate the histograms can be found in Data S1.