Palmitoylation of Gephyrin Controls Receptor Clustering and Plasticity of GABAergic Synapses
Figure 8
Gephyrin palmitoylation is regulated by GABAAR-activity.
(a–e) Primary hippocampal neurons were cultured 14 DIV and exposed to 50 µM bicuculline (BIC) or GABA for 1 h. (b) Representative Western blot shows altered gephyrin palmitoylation in the presence of BIC and GABA. (c) Quantification of gephyrin palmitoylation in neurons treated as in (a). Palmitoylation was normalized to total levels of gephyrin (n = 3). (d) Representative immunoblot of subcellular fractionation of neurons after treatment as in (a). (e) Representative immunostaining of gephyrin and VGAT. Scale bar, 5 µm. Histogram showing quantification of gephyrin cluster size after exposure to bicuculline or GABA compared to control cultures (see a) from three independent cultures. All data are means ± SEM (**p<0.01 using Student's t test).