Palmitoylation of Gephyrin Controls Receptor Clustering and Plasticity of GABAergic Synapses
Figure 7
Subcellular localization of the DHHC-12/gephyrin interaction.
(a) Representative image of a primary hippocampal neuron transfected with DHHC-12–HA (red) immunostained together with the Golgi-marker giantin (green). The higher magnification below shows Golgi localization of DHHC-12 in the cell body and in dendrites as vesicle-like structures. (b) PLA dots (red) demonstrate direct interaction of gephyrin-GFP and DHHC-12–HA in cultured neurons. PLA dots in the inset show gephyrin-GFP and DHHC-12–HA interaction outside gephyrin clusters. Scale bars, 20 µm. (c) Co-immunoprecipitation of gephyrin-myc from HEK293 lysates co-expressing HA-tagged DHHC enzymes as indicated with HA-tag–specific antibodies.