Palmitoylation of Gephyrin Controls Receptor Clustering and Plasticity of GABAergic Synapses
Figure 6
Electrophysiological analysis of GABAergic and glutamatergic mPSCs.
(a) Example traces showing mPSCs recorded from DHHC-12– and DHHS-12–expressing neurons (identified using bicistronic mCherry expression). AMPAR- and GABAAR-mediated mPSCs were distinguished according to fast (AMPAR) and slow (GABAAR) decay kinetics. (b) The zoomed region of a recording from a DHHC-12–expressing neuron illustrates exponential fits of the different decay time constant (gray, AMPAR, τ = 3.8 ms; red, GABAAR, τ = 19.2 ms). (c–f) Quantification of amplitude, frequency, rise times (10–90), and decay time constants of AMPAR- and GABAAR-mediated mPSCs in DHHC-12– or DHHS-12–expressing neurons. Number of quantified events or neurons are given in parentheses.