A Tetraploid Intermediate Precedes Aneuploid Formation in Yeasts Exposed to Fluconazole
Figure 5
Nuclei within trimeras undergo mitotic collapse to form tetraploids.
(A) Time-lapse images of trimera formation and mitotic dynamics. Numbers in fluorescent images denote time in minutes. Also indicated is the percent of trimeras (n = 19) that formed four individual nucleoli (top sequence) and that formed three nucleoli (lower sequence) Scale bars, 5 µm. (B) Violin plots of nuclear DNA content (Hhf1-GFP fluorescence) and SPB number (Tub4-mCherry foci). Colors correspond to measurements of individual nuclei within cells as illustrated below plots: trimeras with two nuclei (purple, n = 56 nuclei), trimeras with four nuclei (pink, n = 24), and trimeras with three nucleoli, one large (green, n = 11) and two small (cyan, n = 22). Gray regions define average fluorescence intensity of 2N and 4N nuclei ±1 standard deviation based on log-phase “no drug” control cells.