A Modular Library of Small Molecule Signals Regulates Social Behaviors in Caenorhabditis elegans
Figure 3
Social and solitary wild-type hermaphrodites are attracted to icas#3, but not to non-indole ascarosides.
(A) Solitary and social wild-type hermaphrodites are not attracted to a physiological ascr#2,3,5 mixture in the spot attraction assays, in contrast to npr-1(ad609) mutant worms (***p<0.0001, unpaired t test with Welch's correction). (B) In the quadrant chemotaxis assay, hermaphrodites from all tested strains are attracted to 1 pM icas#3 and repelled by a physiological mixture of non-indole ascarosides (10 nM ascr#2,3,5), except for npr-1(ad609) mutant worms, which are also attracted to the ascr#2,3,5 blend (chemotaxis after 30 min; for chemotaxis indices at 15 min, see Figure S3C). (C) Dose-dependence of icas#3 attraction for social hermaphrodites in the quadrant chemotaxis assay (Figure 3B,C: *p<0.05, **p<0.01, one-factor ANOVA with Dunnett's post-test). (D) Social wild-type hermaphrodites and npr-1(ad609) mutant worms are attracted to icas#3 in the spot attraction assay (**p<0.001, ***p<0.0001, unpaired t test with Welch's correction).