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Axonal Dynamics of Excitatory and Inhibitory Neurons in Somatosensory Cortex

Figure 7

Cell type specificity of expression in GAD65.eGFP.AAV infected neurons.

(A) Combined eGFP expression and labeling with antibodies against calcium-binding proteins. Left, neurons labeled with GAD65.eGFP.AAV. Middle, neurons labeled with a cocktail of antibodies against the calcium-binding proteins calbindin, calretinin, and parvalbumin. Right, merged images, double-labeled neurons are yellow. (B) Number of neurons labeled by GAD65.eGFP.AAV that were co-localized with calcium binding proteins. (C) Axons of inhibitory neurons in normal cortex. Left, initial imaging session. Right, same region imaged 5 d later.

Figure 7

doi: https://doi.org/10.1371/journal.pbio.1000395.g007