Open Syntaxin Docks Synaptic Vesicles
Figure 5
Syntaxin Is Essential for Synaptic Vesicle Docking
Each row shows two comparisons: total docked vesicles and the distribution of docked vesicles. For total docked vesicles (left), the mean number of docked vesicles per profile was calculated for each synapse (see Table S1 for complete methods and results). Bars show mean and standard error of the mean; *, p-values < 0.001. For vesicle distributions (right), the distance from the dense projection to each docked vesicle was determined, and these measurements were sorted into 33-nm bins. The number of vesicles in each bin was divided by the number of profiles to yield an average number of vesicles per profile in each bin. For both comparisons, only vesicles in profiles containing a dense projection were included.
(A) Shown is a comparison of acetylcholine neurons in wild-type animals and mosaic animals with reduced syntaxin in the GABA and acetylcholine neurons (EG3278). Wild type n = 1 animal, five synapses, and 20 profiles and mosaic n = 1 animal, five synapses, and 24 profiles.
(B) Shown is a comparison of GABA neurons in wild-type animals and mosaic animals with reduced syntaxin in the GABA and acetylcholine neurons (EG3278). Wild type n = 1 animal, four synapses, and 16 profiles and mosaic n = 1 animal, four synapses, and 22 profiles.
(C) Shown is a comparison of acetylcholine neurons in wild-type animals and mosaic animals lacking syntaxin in the GABA neurons (EG3817). Wild type n = 2 animals, ten synapses, and 53 profiles and mosaic n = 2 animals, 11 synapses, and 66 profiles.
(D) Shown is a comparison of GABA neurons in wild-type animals and mosaic animals (EG3817). Wild type n = 2 animals, eight synapses, and 38 profiles and mosaic n = 2 animals, ten synapses, and 51 profiles.