Myc Dynamically and Preferentially Relocates to a Transcription Factory Occupied by Igh
Figure 5
Trans Colocalization of Transcriptionally Active Genes
Double-label RNA FISH in unstimulated B cells to detect Igh (red) and (A) Eif3s6 (green), (B) Uros (green), (C) Igk (green), (D) Igl (green), and (E) and Actb (green). DAPI staining is blue. Scale bar, 2 μm.
(F) The percentage of Myc, Eif3s6, Uros, Igk, Igl, and Actb signals that overlap with an Igh signal. One hundred nuclei were assessed for each case except Uros, for which 70 nuclei were examined. Myc-Igh versus Eif3s6-Igh, p = 0.02; versus Uros-Igh, p = 0.01; versus Igk-Igh, p = 5.9 × 10−4; versus Igl-Igh, p = 5.2 × 10−5; and versus Actb-Igh, p = 1.0 × 10−6.