The Spindle Pole Bodies Facilitate Nuclear Envelope Division during Closed Mitosis in Fission Yeast
Figure 5
NE Division Fails When the SPBs Are Not Positioned at Elongating Spindle Poles
(A) Time-lapse sequence of a Cut11p-GFP α-tubulin-GFP–expressing wild-type cell when both SPBs were severed from the elongating anaphase spindle by laser microsurgery.
(B) Time-lapse sequence of a Cut11p-GFP α-tubulin-GFP–expressing wild-type cell when one SPB was cut off the spindle. Note that the NE is forming the panhandle-shaped protrusions at the sites of contact with the elongating spindle remnants. DIC images at the end of the sequence show normally proceeding septation. Shown are single maximum-intensity reconstructions of z-stacks. Numbers refer to the time, in minutes and seconds.
(C) Time-lapse fluorescence microscopy analyses of the NE and spindle dynamics in Uch2p-GFP α-tubulin-GFP–expressing wild-type, dis1Δ, and Mia1p-overexpressing cells. Formation of membrane tethers in dis1Δ and Mia1p-overexpressing cells is indicated by arrowheads. Shown are single maximum-intensity reconstructions of z-stacks. Numbers refer to the time, in minutes.