The Mother Centriole Plays an Instructive Role in Defining Cell Geometry
Figure 5
In asq2 Cells, Mother Centrioles Are Properly Localized, Whereas Daughters Are Not
(A) If mother centrioles (white) are properly positioned, then the distance between flagellated centrioles in asq2uni1 mutant cells should be much smaller and less variable than that of single-mutant cells.
(B) The distance between flagellated centrioles in biflagellate asq2uni1 cells is much smaller and less variable (mean = 0.89 ± 0.36 μm, n = 85) than in biflagellate asq2 cells (mean = 1.48 ± 0.85 μm, n =88). This difference is highly significant (one-tailed t-test, p < 1.23 e−8)
(C) If mother centrioles (white) are properly localized, then the θcentriole should be much smaller and less variable for flagellated centrioles in asq2uni1 cells than for asq2 cells.
(D) θcentriole for flagellated centrioles in asq2uni1 cells is significantly (one-tailed t-test p < 2.02 e−10) smaller (green lines, mean θcentriole = 32.4 ± 13.1°, n = 60) and less variable than in asq2 cells (grey lines, mean θcentriole = 61.7 ± 32.4°, n = 71).
(E) Flagellated mother centrioles (m; white arrow) are properly localized in asq2uni1 cells, whereas unflagellated daughter centrioles (d; blue arrow) are not. Cells are labeled with anti-acetylated tubulin and centrin antibody (green), anti-Bld10p antibody specific for centrioles (red) and DAPI (blue). Misplacement of nonflagellated daughter centrioles in vfl2uni1 indicates that uni1 does not simply suppress the centriole positioning phenotype of vfl2.