Selective Translational Repression of Truncated Proteins from Frameshift Mutation-Derived mRNAs in Tumors
Figure 5
Truncated Protein Expression from Mutant NMD-Irrelevant MARCKS mRNA
(A) Schematic diagram of construct Q (MARCKS wild-type genomic DNA) and construct R (MARCKS(−2) deleted genomic DNA).
(B) Western blotting (WB) analysis of total protein (30 μg) isolated from transiently transfected cell lines with constructs Q or R. An antibody against FLAG was used as the primary antibody.
(C) Western blotting (WB) analysis of total protein (30 μg) isolated from transiently transfected cell lines with the constructs Q or R was performed using an antibody against MARCKS. Enhanced green fluorescent protein (EGFP) was used as a control for transfection efficiency. Comparable results were obtained in at least two independent experiments. Asterisks (*) denote endogenous MARCKS reacted with anti-MARCKS antibody; Con. denotes the control vector pcDNA3.1(+). The dagger indicates uncharacterized protein that did not interfere with experimental interpretations.