Selective Translational Repression of Truncated Proteins from Frameshift Mutation-Derived mRNAs in Tumors
Figure 2
Western Blotting Analysis of NMD-Sensitive and NMD-Escape Proteins
Truncated proteins were not detected in NMD-sensitive (hRad50 and hMSH6) genes, whereas wild-type proteins were detected in the cell lines containing the wild-type allele. In the NMD-escape hMSH3 gene, wild-type hMSH3 proteins were detected in the cell lines containing the wild-type allele. However, no truncated proteins of hMSH3 were detected in the cell lines with frameshift mutation. GAPDH was used as a loading control. The single asterisk indicates mutation status of each gene: (−1) denotes a 1-bp deletion in the cMNR, (w) denotes no mutation in the cMNR, and (+1) denotes 1-bp insertion in the cMNR. The double asterisk indicates frameshift mutations of the other coding regions present in both alleles of DLD-1 [38]. The dagger indicates that the expected size of the truncated protein is indicated by the arrow.