Intravital Observation of Plasmodium berghei Sporozoite Infection of the Liver
Figure 9
Sporozoite Transmigration Causes Histopathological Changes in the Liver
Mouse livers were removed 2 d (A–D) or 7 d (E–H) after daily infection with P. yoelii by bite of 150 mosquitoes and stained with H&E (A and E) or Masson's trichrome (B and F). Other sections were subjected to immunohistochemistry using mAb PC10 against proliferating cell nuclear antigen (C and G) or mAb HHF35 against smooth muscle actin (D and H). In contrast to the livers fixed after 2 d of infection, in which only a few cells reacted with mAb PC10 and mAb HHF35 (arrows in C and D), livers examined after 7 d of infection showed (E) increased numbers of nonparenchymal cells lining the sinusoids (arrow), (F) a focal deposition of collagen (blue) in some spaces of Disse, (G) large numbers of proliferating nonparenchymal cells and hepatocytes (brown, arrows), and (H) a focal increase in the concentration of smooth muscle actin (brown, arrow).