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Light-Dependent Development of Circadian Gene Expression in Transgenic Zebrafish

Figure 6

Bioluminescence Rhythms Mediated by per3-luc in DD and LD Measured for Six Days

(A) Average plot of bioluminescence rhythms in DD. Animals were entrained in 14:10 LD cycles for 6 d at 22 °C and tested for approximately 6.5 d in DD.

(B) Representative plot of bioluminescence rhythm in DD for an individual.

(C) Average plot of bioluminescence rhythms in 14:10 LD cycles. Animals were entrained in 6 LD cycles at 22 °C prior to the monitoring.

(D) Individual plot of bioluminescence rhythm in LD cycles. For each of DD and LD experiments, two experiments have been performed with essentially the same results. Only one of two experiments is shown for each of DD and LD. The first 12 h of data were deleted from each plot. Black and white bars on top of each plot represent the time when lights were off and on, respectively. Numbers of animals that were averaged is given at top right corner of (A) and (C). Error bars represent SEM in (A) and (C).

Figure 6

doi: https://doi.org/10.1371/journal.pbio.0030034.g006