IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species

Anubhav Dhar; Sucheta Dey; Sanjana Mullick; Nishant Kumar Suman; Maxime C. van Zwam; Nishaant Kumar Palani Balaji; Angana Ghosh; Deepak Nair; Koen van den Dries; Sudarshan Gadadhar; Saravanan Palani

doi:10.1371/journal.pbio.3003832

Loading metrics

Open Access

Peer-reviewed

Update Articles develop a previous PLOS Biology study by providing new mechanistic insight, identifying the biological or physiological significance of the previous findings, or in another way significantly adding to the original article.

See all article types »

IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species

Anubhav Dhar,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Department of Biochemistry, Division of Biological Sciences, Indian Institute of Science, Bengaluru, Karnataka, India
⨯
Sucheta Dey ,

Contributed equally to this work with: Sucheta Dey, Sanjana Mullick, Nishant Kumar Suman, Maxime C. van Zwam, Nishaant Kumar Palani Balaji

Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing

Affiliations Institute for Stem Cell Science and Regenerative Medicine (BRIC-inStem), Bengaluru, Karnataka, India, Regional Centre for Biotechnology (RCB), Faridabad, Haryana, India
⨯
Sanjana Mullick ,

Contributed equally to this work with: Sucheta Dey, Sanjana Mullick, Nishant Kumar Suman, Maxime C. van Zwam, Nishaant Kumar Palani Balaji

Roles Data curation, Formal analysis, Validation, Visualization, Writing – review & editing

Affiliations Institute for Stem Cell Science and Regenerative Medicine (BRIC-inStem), Bengaluru, Karnataka, India, Regional Centre for Biotechnology (RCB), Faridabad, Haryana, India
⨯
Nishant Kumar Suman ,

Contributed equally to this work with: Sucheta Dey, Sanjana Mullick, Nishant Kumar Suman, Maxime C. van Zwam, Nishaant Kumar Palani Balaji

Roles Data curation, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing – review & editing

Affiliation Centre for Neuroscience, Division of Biological Sciences, Indian Institute of Science, Bengaluru, Karnataka, India
⨯
Maxime C. van Zwam ,

Contributed equally to this work with: Sucheta Dey, Sanjana Mullick, Nishant Kumar Suman, Maxime C. van Zwam, Nishaant Kumar Palani Balaji

Roles Data curation, Formal analysis, Methodology, Validation, Visualization, Writing – review & editing

Affiliation Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands
⨯
Nishaant Kumar Palani Balaji ,

Contributed equally to this work with: Sucheta Dey, Sanjana Mullick, Nishant Kumar Suman, Maxime C. van Zwam, Nishaant Kumar Palani Balaji

Roles Data curation, Formal analysis, Validation, Visualization, Writing – review & editing

Affiliation Department of Biochemistry, Division of Biological Sciences, Indian Institute of Science, Bengaluru, Karnataka, India
⨯
Angana Ghosh,

Roles Data curation, Visualization, Writing – review & editing

Affiliation Centre for Neuroscience, Division of Biological Sciences, Indian Institute of Science, Bengaluru, Karnataka, India
⨯
Deepak Nair,

Roles Funding acquisition, Investigation, Supervision, Validation, Writing – review & editing

Affiliation Centre for Neuroscience, Division of Biological Sciences, Indian Institute of Science, Bengaluru, Karnataka, India
⨯
Koen van den Dries,

Roles Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Resources, Supervision, Validation, Visualization, Writing – review & editing

Affiliation Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands
⨯
Sudarshan Gadadhar,

Roles Formal analysis, Funding acquisition, Methodology, Supervision, Investigation, Validation, Visualization, Writing – review & editing

Affiliation Institute for Stem Cell Science and Regenerative Medicine (BRIC-inStem), Bengaluru, Karnataka, India
⨯
Saravanan Palani

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: spalani@iisc.ac.in

Affiliation Department of Biochemistry, Division of Biological Sciences, Indian Institute of Science, Bengaluru, Karnataka, India

https://orcid.org/0000-0002-1893-6777

⨯

IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species

Anubhav Dhar,
Sucheta Dey,
Sanjana Mullick,
Nishant Kumar Suman,
Maxime C. van Zwam,
Nishaant Kumar Palani Balaji,
Angana Ghosh,
Deepak Nair,
Koen van den Dries,
Sudarshan Gadadhar

Published: June 12, 2026
https://doi.org/10.1371/journal.pbio.3003832

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Actins
Is the Subject Area "Actins" applicable to this article?

Thanks for your feedback.
Cell staining
Is the Subject Area "Cell staining" applicable to this article?

Thanks for your feedback.
Schizosaccharomyces pombe
Is the Subject Area "Schizosaccharomyces pombe" applicable to this article?

Thanks for your feedback.
Saccharomyces cerevisiae
Is the Subject Area "Saccharomyces cerevisiae" applicable to this article?

Thanks for your feedback.
Yeast
Is the Subject Area "Yeast" applicable to this article?

Thanks for your feedback.
Neurons
Is the Subject Area "Neurons" applicable to this article?

Thanks for your feedback.
Protein structure
Is the Subject Area "Protein structure" applicable to this article?

Thanks for your feedback.
Hippocampus
Is the Subject Area "Hippocampus" applicable to this article?

Thanks for your feedback.