The RNA-binding protein RBM39 scaffolds an m⁶A-dependent RNA decay complex that destabilizes Tat transcripts and restricts HIV-1 reactivation

Xiaohui Deng; Siyi Xie; Mo Zhou; Quyu Yuan; Shuangxin Wu; Peiming Huang; Minghua Chen; Jianteng Zeng; Pengle Guo; Jie Qin; Cancan Chen; Jiaye Liu; Bingfeng Liu; Xin He; Liqin Sun; Hui Zhang; Linghua Li; Ting Pan

doi:10.1371/journal.pbio.3003486

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Open Access

Peer-reviewed

Research Article

The RNA-binding protein RBM39 scaffolds an m⁶A-dependent RNA decay complex that destabilizes Tat transcripts and restricts HIV-1 reactivation

Xiaohui Deng ,

Contributed equally to this work with: Xiaohui Deng, Siyi Xie, Mo Zhou, Quyu Yuan

Roles Data curation, Methodology, Validation

Affiliations Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat-Sen University, Sun Yat-Sen University, Shenzhen, Guangdong, China, Guangdong Institute of Intelligence Science and Technology, Zhuhai, Guangdong, China
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Siyi Xie ,

Contributed equally to this work with: Xiaohui Deng, Siyi Xie, Mo Zhou, Quyu Yuan

Roles Data curation, Methodology, Project administration

Affiliation Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat-Sen University, Sun Yat-Sen University, Shenzhen, Guangdong, China
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Mo Zhou ,

Contributed equally to this work with: Xiaohui Deng, Siyi Xie, Mo Zhou, Quyu Yuan

Roles Conceptualization, Methodology, Resources, Validation

Affiliation Infectious Disease Center, Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou, China
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Quyu Yuan ,

Contributed equally to this work with: Xiaohui Deng, Siyi Xie, Mo Zhou, Quyu Yuan

Roles Data curation, Formal analysis, Methodology, Validation

Affiliation Institute of Human Virology, Department of Pathogen Biology and Biosecurity, Key Laboratory of Tropical Disease Control of Ministry Education, Guangdong Engineering Research Center for Antimicrobial Agent and Immunotechnology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China
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Shuangxin Wu,

Roles Methodology, Resources, Software

Affiliations Institute of Human Virology, Department of Pathogen Biology and Biosecurity, Key Laboratory of Tropical Disease Control of Ministry Education, Guangdong Engineering Research Center for Antimicrobial Agent and Immunotechnology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China, Medical Research Center, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen, Guangdong, China
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Peiming Huang,

Roles Data curation, Formal analysis, Methodology, Software

Affiliation Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat-Sen University, Sun Yat-Sen University, Shenzhen, Guangdong, China
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Minghua Chen,

Roles Methodology, Software, Validation

Affiliation Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat-Sen University, Sun Yat-Sen University, Shenzhen, Guangdong, China
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Jianteng Zeng,

Roles Data curation, Resources, Validation

Affiliation Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat-Sen University, Sun Yat-Sen University, Shenzhen, Guangdong, China
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Pengle Guo,

Roles Conceptualization, Resources, Software

Affiliation Infectious Disease Center, Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou, China
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Jie Qin,

Roles Methodology, Resources

Affiliation Department of Gastrointestinal Surgery, Shenzhen Third People’s Hospital, The Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen, Guangdong, China
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Cancan Chen,

Roles Funding acquisition, Resources

Affiliation Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China
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Jiaye Liu,

Roles Methodology, Resources

Affiliation School of Public Health, Shenzhen University Health Science Center, Shenzhen, Guangdong, China
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Bingfeng Liu,

Roles Methodology, Resources, Software

Affiliation Institute of Human Virology, Department of Pathogen Biology and Biosecurity, Key Laboratory of Tropical Disease Control of Ministry Education, Guangdong Engineering Research Center for Antimicrobial Agent and Immunotechnology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China
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Xin He,

Roles Resources, Software

Affiliation Institute of Human Virology, Department of Pathogen Biology and Biosecurity, Key Laboratory of Tropical Disease Control of Ministry Education, Guangdong Engineering Research Center for Antimicrobial Agent and Immunotechnology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China
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Liqin Sun ,

Roles Formal analysis, Methodology, Resources

* E-mail: sunliqin888@126.com (LS); zhangh92@mail.sysu.edu.cn (HZ); llheliza@126.com (LL); pant8@mail.sysu.edu.cn (TP)

Affiliation Department Infectious Diseases, National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital, Shenzhen, Guangdong, China
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Hui Zhang ,

Roles Investigation, Project administration, Resources

* E-mail: sunliqin888@126.com (LS); zhangh92@mail.sysu.edu.cn (HZ); llheliza@126.com (LL); pant8@mail.sysu.edu.cn (TP)

Affiliation Institute of Human Virology, Department of Pathogen Biology and Biosecurity, Key Laboratory of Tropical Disease Control of Ministry Education, Guangdong Engineering Research Center for Antimicrobial Agent and Immunotechnology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China
⨯
Linghua Li ,

Roles Project administration, Resources, Software

* E-mail: sunliqin888@126.com (LS); zhangh92@mail.sysu.edu.cn (HZ); llheliza@126.com (LL); pant8@mail.sysu.edu.cn (TP)

Affiliation Infectious Disease Center, Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou, China
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[ ... ],
Ting Pan

Roles Formal analysis, Funding acquisition, Investigation, Project administration, Writing – original draft, Writing – review & editing

* E-mail: sunliqin888@126.com (LS); zhangh92@mail.sysu.edu.cn (HZ); llheliza@126.com (LL); pant8@mail.sysu.edu.cn (TP)

Affiliation Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat-Sen University, Sun Yat-Sen University, Shenzhen, Guangdong, China

https://orcid.org/0000-0002-7106-7312

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The RNA-binding protein RBM39 scaffolds an m⁶A-dependent RNA decay complex that destabilizes Tat transcripts and restricts HIV-1 reactivation

Xiaohui Deng,
Siyi Xie,
Mo Zhou,
Quyu Yuan,
Shuangxin Wu,
Peiming Huang,
Minghua Chen,
Jianteng Zeng,
Pengle Guo,
Jie Qin

Published: November 11, 2025
https://doi.org/10.1371/journal.pbio.3003486

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