Structures of G-protein coupled receptor HCAR1 in complex with Gi1 protein reveal the mechanistic basis for ligand recognition and agonist selectivity

• Xin Pan ,

  Contributed equally to this work with: Xin Pan, Fang Ye, Peiruo Ning, Yiping Yu

  Roles Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Department of Cardiology, Institute of Cardiovascular Disease, Yangzhou Key Lab of Innovation Frontiers in Cardiovascular Disease, Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China

  ⨯
• Fang Ye ,

  Contributed equally to this work with: Xin Pan, Fang Ye, Peiruo Ning, Yiping Yu

  Roles Conceptualization, Data curation, Investigation, Methodology

  Affiliation Kobilka Institute of Innovative Drug Discovery, The Second Affiliated Hospital, Shenzhen Futian Biomedical Innovation R&D Center, School of Medicine, Chinese University of Hong Kong, Shenzhen, China

  ⨯
• Peiruo Ning ,

  Contributed equally to this work with: Xin Pan, Fang Ye, Peiruo Ning, Yiping Yu

  Roles Conceptualization, Data curation, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Kobilka Institute of Innovative Drug Discovery, The Second Affiliated Hospital, Shenzhen Futian Biomedical Innovation R&D Center, School of Medicine, Chinese University of Hong Kong, Shenzhen, China

  ⨯
• Yiping Yu ,

  Contributed equally to this work with: Xin Pan, Fang Ye, Peiruo Ning, Yiping Yu

  Roles Data curation, Investigation, Methodology

  Affiliation Warshel Institute for Computational Biology, School of Medicine, the Chinese University of Hong Kong, Shenzhen, China

  ⨯
• Zhiyi Zhang,

  Roles Investigation

  Affiliation Kobilka Institute of Innovative Drug Discovery, The Second Affiliated Hospital, Shenzhen Futian Biomedical Innovation R&D Center, School of Medicine, Chinese University of Hong Kong, Shenzhen, China

  ⨯
• Jingxuan Wang,

  Roles Investigation

  Affiliation Kobilka Institute of Innovative Drug Discovery, The Second Affiliated Hospital, Shenzhen Futian Biomedical Innovation R&D Center, School of Medicine, Chinese University of Hong Kong, Shenzhen, China

  ⨯
• Geng Chen,

  Roles Investigation

  Affiliation Kobilka Institute of Innovative Drug Discovery, The Second Affiliated Hospital, Shenzhen Futian Biomedical Innovation R&D Center, School of Medicine, Chinese University of Hong Kong, Shenzhen, China

  ⨯
• Zhangsong Wu,

  Roles Investigation

  Affiliation Kobilka Institute of Innovative Drug Discovery, The Second Affiliated Hospital, Shenzhen Futian Biomedical Innovation R&D Center, School of Medicine, Chinese University of Hong Kong, Shenzhen, China

  ⨯
• Chen Qiu,

  Roles Investigation

  Affiliation Kobilka Institute of Innovative Drug Discovery, The Second Affiliated Hospital, Shenzhen Futian Biomedical Innovation R&D Center, School of Medicine, Chinese University of Hong Kong, Shenzhen, China

  ⨯
• Jiancheng Li,

  Roles Investigation

  Affiliation Instrumental Analysis Center, Shenzhen University, Shenzhen, China

  ⨯
• Bangning Chen,

  Roles Investigation

  Affiliation Department of Reagent Research and Development, Shenzhen YHLO Biotech Co.,Ltd., Shenzhen, China

  ⨯
• Lizhe Zhu ,

  * E-mail: yangdu@cuhk.edu.cn (YD); yungkzh@163.com (KG); chungen_qian@hust.edu.cn (CQ); zhulizhe@cuhk.edu.cn (LZ)

  Affiliation Warshel Institute for Computational Biology, School of Medicine, the Chinese University of Hong Kong, Shenzhen, China

  ⨯
• Chungen Qian ,

  Roles Supervision

  * E-mail: yangdu@cuhk.edu.cn (YD); yungkzh@163.com (KG); chungen_qian@hust.edu.cn (CQ); zhulizhe@cuhk.edu.cn (LZ)

  Affiliation Department of Reagent Research and Development, Shenzhen YHLO Biotech Co.,Ltd., Shenzhen, China

  ⨯
• Kaizheng Gong ,

  Roles Funding acquisition, Supervision, Writing – review & editing

  * E-mail: yangdu@cuhk.edu.cn (YD); yungkzh@163.com (KG); chungen_qian@hust.edu.cn (CQ); zhulizhe@cuhk.edu.cn (LZ)

  Affiliation Department of Cardiology, Institute of Cardiovascular Disease, Yangzhou Key Lab of Innovation Frontiers in Cardiovascular Disease, Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China

  ⨯
• Yang Du

  Roles Conceptualization, Funding acquisition, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: yangdu@cuhk.edu.cn (YD); yungkzh@163.com (KG); chungen_qian@hust.edu.cn (CQ); zhulizhe@cuhk.edu.cn (LZ)

  Affiliations Kobilka Institute of Innovative Drug Discovery, The Second Affiliated Hospital, Shenzhen Futian Biomedical Innovation R&D Center, School of Medicine, Chinese University of Hong Kong, Shenzhen, China, Department of Endocrinology, Peking Union Medical College Hospital, Beijing, China

  https://orcid.org/0000-0001-5115-0736

  ⨯