A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution

Shihao Zhou; Qiyu Zhu; Minho Eom; Shilin Fang; Oksana M. Subach; Chen Ran; Jonnathan Singh Alvarado; Praneel S. Sunkavalli; Yuanping Dong; Yangdong Wang; Jiewen Hu; Hanbin Zhang; Zhiyuan Wang; Xiaoting Sun; Tao Yang; Yu Mu; Young-Gyu Yoon; Zengcai V. Guo; Fedor V. Subach; Kiryl D. Piatkevich

doi:10.1371/journal.pbio.3003048

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Peer-reviewed

Methods and Resources report novel methods, substantial improvements to current methodologies, or informational datasets.

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A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution

Shihao Zhou ,

Contributed equally to this work with: Shihao Zhou, Qiyu Zhu, Minho Eom, Shilin Fang

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliations School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China, Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China
⨯
Qiyu Zhu ,

Contributed equally to this work with: Shihao Zhou, Qiyu Zhu, Minho Eom, Shilin Fang

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – review & editing

Affiliations IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing, China, School of Basic Medical Sciences, Tsinghua University, Beijing, China, Tsinghua-Peking Center for Life Sciences, Beijing, China
⨯
Minho Eom ,

Contributed equally to this work with: Shihao Zhou, Qiyu Zhu, Minho Eom, Shilin Fang

Roles Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – review & editing

Affiliation School of Electrical Engineering, KAIST, Daejeon, Republic of Korea
⨯
Shilin Fang ,

Contributed equally to this work with: Shihao Zhou, Qiyu Zhu, Minho Eom, Shilin Fang

Roles Data curation, Formal analysis, Methodology, Software, Validation, Visualization, Writing – review & editing

Affiliation Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China
⨯
Oksana M. Subach,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – review & editing

Affiliation Complex of NBICS Technologies, National Research Center “Kurchatov Institute”, Moscow, Russia
⨯
Chen Ran,

Roles Data curation, Formal analysis, Methodology, Resources, Validation

Affiliation Department of Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California, United States of America
⨯
Jonnathan Singh Alvarado,

Roles Data curation, Formal analysis, Methodology, Visualization

Affiliation Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Praneel S. Sunkavalli,

Roles Data curation, Formal analysis, Methodology, Visualization

Affiliation Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Yuanping Dong,

Roles Data curation, Formal analysis, Methodology, Visualization

Affiliations School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China
⨯
Yangdong Wang,

Roles Investigation, Methodology

Affiliations School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China, Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China
⨯
Jiewen Hu,

Roles Formal analysis, Validation

Affiliation Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China
⨯
Hanbin Zhang,

Roles Methodology

Affiliations School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China, Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China
⨯
Zhiyuan Wang,

Roles Formal analysis

Affiliations Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China, University of Chinese Academy of Sciences, Beijing, China
⨯
Xiaoting Sun,

Roles Methodology

Affiliations School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China, Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China
⨯
Tao Yang,

Roles Resources, Supervision

Affiliations School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China
⨯
Yu Mu ,

Roles Conceptualization, Resources, Supervision, Writing – review & editing

* E-mail: kiryl.piatkevich@westlake.edu.cn (KDP); subach_fv@nrcki.ru (FVS); guozengcai@mail.tsinghua.edu.cn (ZVG); younggyu.yoon@gmail.com (YGY); my@ion.ac.cn (YM)

Affiliations Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China, University of Chinese Academy of Sciences, Beijing, China

https://orcid.org/0000-0001-9297-4854

⨯
Young-Gyu Yoon ,

Roles Resources, Supervision, Writing – review & editing

* E-mail: kiryl.piatkevich@westlake.edu.cn (KDP); subach_fv@nrcki.ru (FVS); guozengcai@mail.tsinghua.edu.cn (ZVG); younggyu.yoon@gmail.com (YGY); my@ion.ac.cn (YM)

Affiliations School of Electrical Engineering, KAIST, Daejeon, Republic of Korea, KAIST Institute for Health Science and Technology, Daejeon, Republic of Korea, Department of Semiconductor System Engineering, KAIST, Daejeon, Republic of Korea
⨯
Zengcai V. Guo ,

Roles Resources, Supervision, Writing – review & editing

* E-mail: kiryl.piatkevich@westlake.edu.cn (KDP); subach_fv@nrcki.ru (FVS); guozengcai@mail.tsinghua.edu.cn (ZVG); younggyu.yoon@gmail.com (YGY); my@ion.ac.cn (YM)

Affiliations IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing, China, School of Basic Medical Sciences, Tsinghua University, Beijing, China, Tsinghua-Peking Center for Life Sciences, Beijing, China
⨯
Fedor V. Subach ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Supervision, Visualization, Writing – review & editing

* E-mail: kiryl.piatkevich@westlake.edu.cn (KDP); subach_fv@nrcki.ru (FVS); guozengcai@mail.tsinghua.edu.cn (ZVG); younggyu.yoon@gmail.com (YGY); my@ion.ac.cn (YM)

Affiliation Complex of NBICS Technologies, National Research Center “Kurchatov Institute”, Moscow, Russia
⨯
[ ... ],
Kiryl D. Piatkevich

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing

* E-mail: kiryl.piatkevich@westlake.edu.cn (KDP); subach_fv@nrcki.ru (FVS); guozengcai@mail.tsinghua.edu.cn (ZVG); younggyu.yoon@gmail.com (YGY); my@ion.ac.cn (YM)

Affiliations School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China, Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, China

https://orcid.org/0000-0002-7777-9468

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A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution

Shihao Zhou,
Qiyu Zhu,
Minho Eom,
Shilin Fang,
Oksana M. Subach,
Chen Ran,
Jonnathan Singh Alvarado,
Praneel S. Sunkavalli,
Yuanping Dong,
Yangdong Wang

Published: April 29, 2025
https://doi.org/10.1371/journal.pbio.3003048

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