A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist

• Jérémie Le Pen ,

  Contributed equally to this work with: Jérémie Le Pen, Gabrielle Paniccia

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: jlepen@rockefeller.edu (JLP); ricec@rockefeller.edu (CMR)

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  https://orcid.org/0000-0001-7025-9526

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• Gabrielle Paniccia ,

  Contributed equally to this work with: Jérémie Le Pen, Gabrielle Paniccia

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Validation, Visualization, Writing – review & editing

  Current address: Elya Laboratory, Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States of America

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Volker Kinast ,

  Roles Investigation, Writing – review & editing

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliations Department of Medical Microbiology and Virology, Carl von Ossietzky University Oldenburg, Oldenburg, Germany, Department for Molecular and Medical Virology, Faculty of Medicine, Ruhr University Bochum, Bochum, Germany

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• Marcela Moncada-Velez ,

  Roles Investigation

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation St Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York, United States of America

  ⨯
• Alison W. Ashbrook ,

  Roles Investigation, Writing – review & editing

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

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• Michael Bauer ,

  Roles Investigation, Writing – review & editing

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

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• H.-Heinrich Hoffmann ,

  Roles Investigation

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

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• Ana Pinharanda ,

  Roles Investigation, Writing – review & editing

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Department of Biological Sciences, Columbia University, New York, New York, United States of America

  ⨯
• Inna Ricardo-Lax ,

  Roles Investigation, Writing – review & editing

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Ansgar F. Stenzel ,

  Roles Investigation

  Current address: Department of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, Germany

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Edwin A. Rosado-Olivieri ,

  Roles Investigation

  Current address: Department of Molecular Pathobiology, New York University, New York, United States of America

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Laboratory of Synthetic Embryology, The Rockefeller University, New York, New York, United States of America

  ⨯
• Kenneth H. Dinnon III,

  Roles Investigation

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• William C. Doyle,

  Roles Investigation

  Current address: Laboratory of Host-Pathogen Biology, The Rockefeller University, New York, New York, United States of America

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Catherine A. Freije,

  Roles Investigation

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Seon-Hui Hong,

  Roles Investigation

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Danyel Lee,

  Roles Investigation

  Affiliations St Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York, United States of America, Laboratory of Human Genetics of Infectious Diseases, Necker Branch, INSERM U1163, Paris, France, Paris Cité University, Imagine Institute, Paris, France

  ⨯
• Tyler Lewy,

  Roles Investigation

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Joseph M. Luna,

  Roles Investigation

  Current address: Department of Biochemistry and Center for RNA Science and Therapeutics, Case Western Reserve University, Cleveland, Ohio, United States of America

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Avery Peace,

  Roles Investigation

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Carltin Schmidt,

  Roles Investigation

  Current address: Medical Faculty, Heinrich Heine University Dusseldorf, Germany

  Affiliation St Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York, United States of America

  ⨯
• William M. Schneider,

  Roles Investigation, Writing – review & editing

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Roni Winkler,

  Roles Investigation

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Elaine Z. Yip,

  Roles Investigation

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Chloe Larson,

  Roles Resources

  Affiliation Fisher Drug Discovery Resource Center, The Rockefeller University, New York, New York, United States of America

  ⨯
• Timothy McGinn,

  Roles Resources

  Affiliation Fisher Drug Discovery Resource Center, The Rockefeller University, New York, New York, United States of America

  ⨯
• Miriam-Rose Menezes,

  Roles Resources

  Affiliation Fisher Drug Discovery Resource Center, The Rockefeller University, New York, New York, United States of America

  ⨯
• Lavoisier Ramos-Espiritu,

  Roles Resources

  Affiliation Fisher Drug Discovery Resource Center, The Rockefeller University, New York, New York, United States of America

  ⨯
• Priyam Banerjee,

  Roles Resources

  Affiliation Bio-Imaging Resource Center, The Rockefeller University, New York, New York, United States of America

  ⨯
• John T. Poirier,

  Roles Investigation

  Affiliation Laura and Isaac Perlmutter Cancer Center, New York University Grossman School of Medicine, NYU Langone Health, New York, New York, United States of America

  ⨯
• Francisco J. Sànchez-Rivera,

  Roles Investigation

  Affiliations Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

  ⨯
• Aurélie Cobat,

  Roles Formal analysis

  Affiliations St Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York, United States of America, Laboratory of Human Genetics of Infectious Diseases, Necker Branch, INSERM U1163, Paris, France, Paris Cité University, Imagine Institute, Paris, France

  ⨯
• Qian Zhang,

  Roles Investigation

  Affiliations St Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York, United States of America, Laboratory of Human Genetics of Infectious Diseases, Necker Branch, INSERM U1163, Paris, France, Paris Cité University, Imagine Institute, Paris, France

  ⨯
• Jean-Laurent Casanova,

  Roles Supervision

  Affiliations St Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York, United States of America, Laboratory of Human Genetics of Infectious Diseases, Necker Branch, INSERM U1163, Paris, France, Paris Cité University, Imagine Institute, Paris, France, Department of Pediatrics, Necker Hospital for Sick Children, Paris, France, Howard Hughes Medical Institute, New York, New York, United States of America

  ⨯
• Thomas S. Carroll,

  Roles Data curation, Formal analysis, Methodology, Resources, Software

  Affiliation Bioinformatics Resource Center, The Rockefeller University, New York, New York, United States of America

  ⨯
• J. Fraser Glickman,

  Roles Resources, Supervision

  Affiliation Fisher Drug Discovery Resource Center, The Rockefeller University, New York, New York, United States of America

  ⨯
• Eleftherios Michailidis ,

  Roles Conceptualization, Funding acquisition, Investigation

  Current address: Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia, United States of America

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Brandon Razooky ,

  Roles Conceptualization, Investigation

  Current address: National Resilience, Inc., La Jolla, California, United States of America

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
• Margaret R. MacDonald ,

  Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

  ⨯
•  [ ... ],
• Charles M. Rice

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

  * E-mail: jlepen@rockefeller.edu (JLP); ricec@rockefeller.edu (CMR)

  ¶‡ VK, MM-V, AWA, MB, H-HH, AP, IR-L, AFS, and EAR-O also contributed equally to this work. EM, BR, MRM, and CMR also contributed equally to this work.

  Affiliation Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, United States of America

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