Engineering of a high-fidelity Cas12a nuclease variant capable of allele-specific editing

• Jingjing Wei,

  Roles Data curation, Investigation

  Affiliations Department of Cardiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China, Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, School of Life Sciences, Shanghai Engineering Research Center of Industrial Microorganisms, Fudan University, Shanghai, China

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• Jingtong Liu,

  Roles Formal analysis

  Affiliation Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, School of Life Sciences, Shanghai Engineering Research Center of Industrial Microorganisms, Fudan University, Shanghai, China

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• Ziwen Wang,

  Roles Data curation

  Affiliation State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China

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• Yuan Yang,

  Roles Formal analysis

  Affiliation Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, School of Life Sciences, Shanghai Engineering Research Center of Industrial Microorganisms, Fudan University, Shanghai, China

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• Yuwen Tian,

  Roles Data curation

  Affiliation Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, School of Life Sciences, Shanghai Engineering Research Center of Industrial Microorganisms, Fudan University, Shanghai, China

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• Shengzhou Wang,

  Roles Formal analysis

  Affiliation Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, School of Life Sciences, Shanghai Engineering Research Center of Industrial Microorganisms, Fudan University, Shanghai, China

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• Bao-Qing Gao,

  Roles Formal analysis

  Affiliations Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China, Center for Molecular Medicine, Children’s Hospital, Fudan University, Shanghai, China, Shanghai Key Laboratory of Medical Epigenetics, International Laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China

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• Song Gao,

  Roles Supervision

  Affiliation State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China

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• Li Yang,

  Roles Supervision

  Affiliations Center for Molecular Medicine, Children’s Hospital, Fudan University, Shanghai, China, Shanghai Key Laboratory of Medical Epigenetics, International Laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China

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• Junnan Tang ,

  Roles Supervision

  * E-mail: fcctangjn@zzu.edu.cn (JT); ymw@fudan.edu.cn (YW)

  Affiliation Department of Cardiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

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• Yongming Wang

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: fcctangjn@zzu.edu.cn (JT); ymw@fudan.edu.cn (YW)

  Affiliations Department of Cardiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China, Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, School of Life Sciences, Shanghai Engineering Research Center of Industrial Microorganisms, Fudan University, Shanghai, China

  https://orcid.org/0000-0001-8269-5296

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