Combining nanobody labeling with STED microscopy reveals input-specific and layer-specific organization of neocortical synapses

Yeasmin Akter; Grace Jones; Grant J. Daskivich; Victoria Shifflett; Karina J. Vargas; Martin Hruska

doi:10.1371/journal.pbio.3002649

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Combining nanobody labeling with STED microscopy reveals input-specific and layer-specific organization of neocortical synapses

Yeasmin Akter ,

Contributed equally to this work with: Yeasmin Akter, Grace Jones

Roles Data curation, Formal analysis, Investigation, Writing – original draft, Writing – review & editing

Affiliation Department of Neuroscience, Rockefeller Neuroscience Institute, West Virginia University, Morgantown, West Virginia, United States of America
⨯
Grace Jones ,

Contributed equally to this work with: Yeasmin Akter, Grace Jones

Roles Data curation, Formal analysis, Investigation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Department of Neuroscience, Rockefeller Neuroscience Institute, West Virginia University, Morgantown, West Virginia, United States of America
⨯
Grant J. Daskivich,

Roles Data curation, Methodology, Writing – review & editing

Affiliation Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
⨯
Victoria Shifflett,

Roles Data curation, Formal analysis

Affiliation Department of Neuroscience, Rockefeller Neuroscience Institute, West Virginia University, Morgantown, West Virginia, United States of America
⨯
Karina J. Vargas,

Roles Data curation, Methodology, Resources, Supervision, Writing – review & editing

Affiliation Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
⨯
Martin Hruska

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing

* E-mail: martin.hruska@hsc.wvu.edu

Affiliation Department of Neuroscience, Rockefeller Neuroscience Institute, West Virginia University, Morgantown, West Virginia, United States of America

https://orcid.org/0000-0003-3186-770X

⨯

Combining nanobody labeling with STED microscopy reveals input-specific and layer-specific organization of neocortical synapses

Yeasmin Akter,
Grace Jones,
Grant J. Daskivich,
Victoria Shifflett,
Karina J. Vargas,
Martin Hruska

Published: April 4, 2025
https://doi.org/10.1371/journal.pbio.3002649

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