Production of the antidepressant orcinol glucoside in Yarrowia lipolytica with yields over 6,400-fold higher than plant extraction

Bihuan Chen; Xiaonan Liu; Yina Wang; Jie Bai; Xiangyu Liu; Guisheng Xiang; Wei Liu; Xiaoxi Zhu; Jian Cheng; Lina Lu; Guanghui Zhang; Ge Zhang; Zongjie Dai; Shuhui Zi; Shengchao Yang; Huifeng Jiang

doi:10.1371/journal.pbio.3002131

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Open Access

Peer-reviewed

Research Article

Production of the antidepressant orcinol glucoside in Yarrowia lipolytica with yields over 6,400-fold higher than plant extraction

Bihuan Chen ,

Contributed equally to this work with: Bihuan Chen, Xiaonan Liu, Yina Wang, Jie Bai

Roles Conceptualization, Formal analysis, Investigation, Methodology, Writing – original draft

Affiliations College of Agronomy and Biotechnology, National and Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, China, Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, National Center of Technology Innovation for Synthetic Biology, Tianjin, China, Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, China
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Xiaonan Liu ,

Contributed equally to this work with: Bihuan Chen, Xiaonan Liu, Yina Wang, Jie Bai

Roles Conceptualization, Writing – original draft

Affiliations Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, National Center of Technology Innovation for Synthetic Biology, Tianjin, China, University of Chinese Academy of Sciences, Beijing, China
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Yina Wang ,

Contributed equally to this work with: Bihuan Chen, Xiaonan Liu, Yina Wang, Jie Bai

Roles Formal analysis, Methodology

Affiliations College of Agronomy and Biotechnology, National and Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, China, Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, China
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Jie Bai ,

Contributed equally to this work with: Bihuan Chen, Xiaonan Liu, Yina Wang, Jie Bai

Roles Conceptualization, Software

Affiliations Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, National Center of Technology Innovation for Synthetic Biology, Tianjin, China, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
⨯
Xiangyu Liu,

Roles Methodology, Validation

Affiliations College of Agronomy and Biotechnology, National and Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, China, Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, China
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Guisheng Xiang,

Roles Formal analysis, Software, Validation

Affiliations College of Agronomy and Biotechnology, National and Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, China, Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, China
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Wei Liu,

Roles Investigation, Validation

Affiliations Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, National Center of Technology Innovation for Synthetic Biology, Tianjin, China, College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, China
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Xiaoxi Zhu,

Roles Investigation, Validation

Affiliations Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, National Center of Technology Innovation for Synthetic Biology, Tianjin, China, University of Chinese Academy of Sciences, Beijing, China
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Jian Cheng,

Roles Formal analysis, Software, Validation

Affiliations Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, National Center of Technology Innovation for Synthetic Biology, Tianjin, China
⨯
Lina Lu,

Roles Investigation

Affiliations Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, National Center of Technology Innovation for Synthetic Biology, Tianjin, China
⨯
Guanghui Zhang,

Roles Investigation, Supervision

Affiliations College of Agronomy and Biotechnology, National and Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, China, Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, China
⨯
Ge Zhang,

Roles Investigation

Affiliation National Center of Technology Innovation for Synthetic Biology, Tianjin, China
⨯
Zongjie Dai,

Roles Investigation

Affiliation National Center of Technology Innovation for Synthetic Biology, Tianjin, China
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Shuhui Zi ,

Roles Funding acquisition, Investigation

* E-mail: 258437889@qq.com (SZ); shengchaoyang@163.com (SY); jiang_hf@tib.cas.cn (HJ)

Affiliations College of Agronomy and Biotechnology, National and Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, China, Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, China
⨯
Shengchao Yang ,

Roles Funding acquisition, Supervision

* E-mail: 258437889@qq.com (SZ); shengchaoyang@163.com (SY); jiang_hf@tib.cas.cn (HJ)

Affiliations College of Agronomy and Biotechnology, National and Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, China, Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, China
⨯
[ ... ],
Huifeng Jiang

Roles Conceptualization, Funding acquisition, Writing – original draft

* E-mail: 258437889@qq.com (SZ); shengchaoyang@163.com (SY); jiang_hf@tib.cas.cn (HJ)

Affiliations Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, National Center of Technology Innovation for Synthetic Biology, Tianjin, China

https://orcid.org/0000-0002-3757-7896

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Production of the antidepressant orcinol glucoside in Yarrowia lipolytica with yields over 6,400-fold higher than plant extraction

Bihuan Chen,
Xiaonan Liu,
Yina Wang,
Jie Bai,
Xiangyu Liu,
Guisheng Xiang,
Wei Liu,
Xiaoxi Zhu,
Jian Cheng,
Lina Lu

Published: June 6, 2023
https://doi.org/10.1371/journal.pbio.3002131

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