Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage

• Li Jianwei ,

  Contributed equally to this work with: Li Jianwei, Chacko Jobichen

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

  Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore

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• Chacko Jobichen ,

  Contributed equally to this work with: Li Jianwei, Chacko Jobichen

  Roles Data curation, Formal analysis, Investigation, Validation, Writing – review & editing

  Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore

  ⨯
• Satoru Machida,

  Roles Data curation, Formal analysis, Investigation, Validation, Writing – review & editing

  Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore

  ⨯
• Sun Meng,

  Roles Investigation

  Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore

  ⨯
• Randy J. Read,

  Roles Investigation

  Affiliation Department of Haematology, University of Cambridge, Cambridge, United Kingdom

  ⨯
• Chen Hongying,

  Roles Investigation

  Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore

  ⨯
• Shi Jian,

  Roles Investigation

  Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore

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• Yuren Adam Yuan †,

  † Deceased.

  Roles Conceptualization, Methodology, Project administration, Supervision

  Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore

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• J. Sivaraman

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Project administration, Supervision, Validation, Writing – review & editing

  * E-mail: dbsjayar@nus.edu.sg

  Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore

  https://orcid.org/0000-0001-9781-5326

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