Identification of SaCas9 orthologs containing a conserved serine residue that determines simple NNGG PAM recognition

• Shuai Wang ,

  Contributed equally to this work with: Shuai Wang, Chen Tao

  Roles Investigation, Visualization

  Affiliation State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, China

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• Chen Tao ,

  Contributed equally to this work with: Shuai Wang, Chen Tao

  Roles Investigation, Visualization

  Affiliation State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, China

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• Huilin Mao,

  Roles Investigation

  Affiliation State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, China

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• Linghui Hou,

  Roles Investigation

  Affiliation State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, China

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• Yao Wang,

  Roles Investigation

  Affiliation State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, China

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• Tao Qi,

  Roles Formal analysis

  Affiliation State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, China

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• Yuan Yang,

  Roles Formal analysis

  Affiliation State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, China

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• Sang-Ging Ong,

  Roles Writing – review & editing

  Affiliations Department of Pharmacology, University of Illinois College of Medicine, Chicago, Illinois, United States of America, Division of Cardiology, Department of Medicine, University of Illinois College of Medicine, Chicago, Illinois, United States of America

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• Shijun Hu ,

  Roles Supervision

  * E-mail: shijunhu@suda.edu.cn (SH); renjiec@seu.edu.cn (RC); ymw@fudan.edu.cn (YW)

  Affiliation Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Suzhou Medical College, Soochow University, Suzhou, China

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• Renjie Chai ,

  Roles Supervision

  * E-mail: shijunhu@suda.edu.cn (SH); renjiec@seu.edu.cn (RC); ymw@fudan.edu.cn (YW)

  Affiliations State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, China, Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, China

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• Yongming Wang

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft

  * E-mail: shijunhu@suda.edu.cn (SH); renjiec@seu.edu.cn (RC); ymw@fudan.edu.cn (YW)

  Affiliations State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, China, Shanghai Engineering Research Center of Industrial Microorganisms, Shanghai, China

  https://orcid.org/0000-0001-8269-5296

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