DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes

• Akihiro Kuno ,

  Contributed equally to this work with: Akihiro Kuno, Yoshihisa Ikeda, Shinya Ayabe

  Roles Conceptualization, Funding acquisition, Investigation, Methodology, Software, Writing – original draft

  * E-mail: akuno@md.tsukuba.ac.jp (AK); konezumi@md.tsukuba.ac.jp (SM)

  Affiliations Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan, Ph.D Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan

  https://orcid.org/0000-0002-4674-6882

  ⨯
• Yoshihisa Ikeda ,

  Contributed equally to this work with: Akihiro Kuno, Yoshihisa Ikeda, Shinya Ayabe

  Roles Investigation, Writing – original draft

  Affiliations Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan, Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Shinya Ayabe ,

  Contributed equally to this work with: Akihiro Kuno, Yoshihisa Ikeda, Shinya Ayabe

  Roles Conceptualization, Project administration, Writing – original draft

  Affiliation Experimental Animal Division, RIKEN BioResource Research Center, Tsukuba, Japan

  https://orcid.org/0000-0003-2737-5315

  ⨯
• Kanako Kato,

  Roles Investigation

  Affiliation Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Kotaro Sakamoto,

  Roles Software, Writing – original draft

  Affiliations Ph.D Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan, Department of Computer Science, University of Tsukuba, Tsukuba, Japan

  https://orcid.org/0000-0003-0007-3894

  ⨯
• Sayaka R. Suzuki,

  Roles Data curation, Writing – original draft

  Affiliations Ph.D Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan, Bioinformatics Laboratory, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Kento Morimoto,

  Roles Investigation

  Affiliation Doctoral Program in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan

  ⨯
• Arata Wakimoto,

  Roles Investigation

  Affiliations Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan, Ph.D Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan

  ⨯
• Natsuki Mikami,

  Roles Investigation, Resources

  Affiliation Ph.D Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan

  ⨯
• Miyuki Ishida,

  Roles Resources

  Affiliation Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Natsumi Iki,

  Roles Resources

  Affiliation Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Yuko Hamada,

  Roles Resources

  Affiliation Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Megumi Takemura,

  Roles Investigation, Resources

  Affiliations Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan, Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Yoko Daitoku,

  Roles Resources

  Affiliation Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Yoko Tanimoto,

  Roles Methodology, Resources

  Affiliation Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Tra Thi Huong Dinh,

  Roles Investigation

  Affiliation Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  https://orcid.org/0000-0003-1705-3865

  ⨯
• Kazuya Murata,

  Roles Conceptualization, Validation

  Affiliations Ph.D Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan, Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  https://orcid.org/0000-0002-3328-069X

  ⨯
• Michito Hamada,

  Roles Resources

  Affiliations Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan, Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  https://orcid.org/0000-0002-4737-1007

  ⨯
• Masafumi Muratani,

  Roles Investigation

  Affiliation Department of Genome Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Atsushi Yoshiki,

  Roles Funding acquisition, Project administration

  Affiliation Experimental Animal Division, RIKEN BioResource Research Center, Tsukuba, Japan

  https://orcid.org/0000-0002-9450-5151

  ⨯
• Fumihiro Sugiyama,

  Roles Conceptualization, Project administration, Writing – review & editing

  Affiliation Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
• Satoru Takahashi,

  Roles Funding acquisition, Project administration, Writing – review & editing

  Affiliations Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan, Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  ⨯
•  [ ... ],
• Seiya Mizuno

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – original draft

  * E-mail: akuno@md.tsukuba.ac.jp (AK); konezumi@md.tsukuba.ac.jp (SM)

  Affiliation Laboratory Animal Resource Center, Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan

  https://orcid.org/0000-0002-6740-5817

  ⨯
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