The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity

• Kevin J. Forsberg ,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: kevin.forsberg@utsouthwestern.edu

  Current address: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

  Affiliations Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America, Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

  https://orcid.org/0000-0002-1545-8925

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• Danica T. Schmidtke,

  Roles Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – review & editing

  Affiliation Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

  https://orcid.org/0000-0001-9509-6188

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• Rachel Werther,

  Roles Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – review & editing

  Affiliation Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

  https://orcid.org/0000-0002-3058-1550

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• Ruben V. Uribe,

  Roles Investigation

  Affiliation Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark

  https://orcid.org/0000-0002-9800-0409

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• Deanna Hausman,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

  https://orcid.org/0000-0003-0914-5022

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• Morten O. A. Sommer,

  Roles Supervision

  Affiliation Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark

  https://orcid.org/0000-0003-4005-5674

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• Barry L. Stoddard,

  Roles Data curation, Funding acquisition, Investigation, Project administration, Resources, Supervision, Visualization, Writing – review & editing

  Affiliation Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

  ⨯
• Brett K. Kaiser,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing

  Affiliations Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America, Department of Biology, Seattle University, Seattle, Washington, United States of America

  ⨯
• Harmit S. Malik

  Roles Funding acquisition, Project administration, Resources, Supervision, Visualization, Writing – review & editing

  Affiliations Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America, Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

  https://orcid.org/0000-0001-6005-0016

  ⨯